Visualization and quantification of cementoblast calcification via alizarin red staining

Autor/Urheber:

Bhargava, S.; Jankowski, J.; Merckelbach, E.; Roth, C.E.; Craveiro, R.B.; Wolf, M.

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https://www.dewiki.de/b/372cad

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Beschreibung:

Figure 5. Visualization and quantification of cementoblast calcification via alizarin red staining. Cementoblasts were incubated for 7 days with 6 different calcification conditions: the first, 1.9 mmol L−1 of phosphate, the second, 2.5 mmol L−1 calcium, the third, 1.3 mmol L−1 phosphate and 0.2 mmol L−1 calcium added to DMEM containing 2.5% FCS. The fourth condition was 75 µmol L−1 ascorbic acid, 10 mmol L−1 β-glycerolphosphate, and 10 nmol L−1 dexamethasone, the fifth, 75 µmol L−1 ascorbic acid, 12.5 mmol L−1 β-glycerolphosphate, and 5 nmol L−1 dexamethasone and the sixth, 75 µmol L−1 ascorbic acid, 7.5 mmol L−1 β-glycerolphosphate, and 5 nmol L−1 dexamethasone added to DMEM containing 5% FCS. The cells were stained by alizarin red staining and imaged. Shown are the representative images of alizarin red staining from conditions 1–3 (A) and conditions 4–6 (C), and the quantification of the percentage of stained area of condition 1–3 (B) and condition 4–6 (D). Data are shown as means ± SEM (n = 9). *** p ≤ 0.001 and **** p ≤ 0.0001 compared with the control based on one-way ANOVA. Bonferroni’s multiple comparisons were used as a post-test.

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CC BY 4.0

Credit:

https://www.mdpi.com/1422-0067/24/18/13829 Bhargava, S.; Jankowski, J.; Merckelbach, E.; Roth, C.E.; Craveiro, R.B.; Wolf, M. Development, Establishment, and Validation of a Model for the Mineralization of Periodontium Remodelling Cells: Cementoblasts. Int. J. Mol. Sci. 2023, 24, 13829. https://doi.org/10.3390/ijms241813829

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