Gel shift assay


Autor/Urheber:
Ganaaaa at en.wikipedia
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Beschreibung:
Lane 1 is a negative control, and contains only DNA. Lane 2 contains protein as well as a DNA fragment that,is based on its sequence, does not interact. Lane 3 contains protein and a DNA fragment that does react; the resulting complex is larger, heavier, and slower-moving. The pattern shown in lane 3 is the one that would result if all the DNA were bound and no dissociation of complex occurred during electrophoresis. When these conditions are not met a second band might be seen in lane 3 reflecting the presence of free DNA or the dissociation of the DNA-protein complex. Created by user Ganaaaa 9-30-2005. I, the creator of this image, hereby release it into the public domain. This applies worldwide.
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Public domain
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Transferred from en.wikipedia by Ronhjones
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